Study of pharmacokinetics in the chemotherapeutic transscleral delivery into the eye globe for the retinoblastoma treatment
Project supervisor:
doc. RNDr. Zuzana Bosáková, CSc. (bosakova@natur.cuni.cz), RNDr. Jakub Širc, Ph.D.
Abstract
Retinoblastoma (Rb) is the most frequent primary intraocular malignant tumor in childhood, which affects 1 per 15,000 – 20,000 newborns. Standard clinical treatment includes local adjuvant therapy treatment followed by systemic administration of chemotherapeutics (CTX). However, closely half of the affected eyes must be enucleated. New ways of CTX local administration using polymeric carriers, increasing CTX concentration in target cells and minimizing serious adverse effects connected with systemic chemotherapy, is being investigated by Institute of Macromolecular Chemistry AS CR, v. v. i. and 2nd Faculty of Medicine, Charles University. The aim of the proposed project is to develop methodology based on liquid chromatography coupled to mass spectrometry for determination of CTX in biological materials; blood plasma, sclera and vitreous. The kinetics of sorption and desorption of CTX to the polymeric carriers will be studied using this method as well, the consequences between polymer structure, drug release and pharmacokinetics in in vivo conditions will be examined.
Deadline is closed