The malignancy of solid cancer is mainly caused by the ability of tumor cells to form metastases. The crucial step during metastasis is the invasion of the cancer cells through the ECM. To achieve this, cancer cells can utilize the protease-dependent mesenchymal invasion mode or more recently discover the amoeboid mode that relies on enhanced cell contractility. All modes of cancer cell invasiveness are interconvertible and could be employed by cancer cells in combination. A great deal of effort of the world wide scientific community has been devoted to studying various aspects of cell invasion and migration. However, despite all the effort, the so far incomplete understanding of the plasticity of cancer cells’ invasiveness precluded successful development of clinically usable anti-metastatic treatment strategies.
The project aims to analyze various aspect of cancer cell invasiveness and to test migrastatic potential of approved drugs as well as other promising candidates. The successful candidate should have experience in mammalian cell cultivation techniques and basic fluorescence microscopy. Experience with live-cell microscopy and molecular cloning are of further advantage.