The malignancy of solid cancer is mainly caused by the ability of tumor cells to form metastases. The crucial step during metastasis is the invasion of the cancer cells through the ECM. To achieve this, cancer cells can utilize the protease-dependent mesenchymal invasion mode or more recently discover the amoeboid mode that relies on enhanced cell contractility. All modes of cancer cell invasiveness are interconvertible and could be employed by cancer cells in combination. A great deal of effort of the world wide scientific community has been devoted to studying various aspects of cell invasion and migration. However, despite all the effort, the so far incomplete understanding of the plasticity of cancer cells’ invasiveness precluded successful development of clinically usable anti-metastatic treatment strategies.
The project aims to analyze various aspect of cancer cell invasiveness and in collaboration with 1st Faculty of Medicine to test migrastatic potential of newly developed anti-cancer drugs. The successful candidate should have experience in mammalian cell cultivation techniques and basic fluorescence microscopy. Experience with live-cell microscopy and molecular cloning are of further advantage.
Koudelková L, Pataki AC, Tolde O, Pavlik V, Nobis M, Gemperle J, Anderson K, Brábek J, Rosel D. Novel FRET-Based Src Biosensor Reveals Mechanisms of Src Activation and Its Dynamics in Focal Adhesions. Cell Chem Biol. 2018 Nov 13. pii: S2451-9456(18)30384-2.
Gemperle J, Dibus M, Koudelková L, Rosel D, Brábek J. The interaction of p130Cas with PKN3 promotes malignant growth. Mol Oncol. 2018 Nov 13. doi: 10.1002/1878-0261.12401.
Čermák V, Gandalovičová A, Merta L, Fučíková J, Špíšek R, Rösel D, Brábek J. RNA-seq of macrophages of amoeboid or mesenchymal migratory phenotype due to specific structure of environment. Sci Data. 2018 Oct 2;5:180198.
Tolde O, Gandalovičová A, Křížová A, Veselý P, Chmelík R, Rosel D, Brábek J. Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion. Sci Rep. 2018 Aug 13;8(1):12020.
Gandalovičová A, Rosel D, Fernandes M, Veselý P, Heneberg P, Čermák V, Petruželka L, Kumar S, Sanz-Moreno V, Brábek J. Migrastatics-Anti-metastatic and Anti-invasion Drugs: Promises and Challenges. Trends Cancer. 2017 Jun;3(6):391-406.
GACR (18-15684J): 2018-2020 The role of matrix metalloproteinases and vimentin cooperation in cancer cell invadopodia function.
MSMT: 2018-2022 Center of Tumor Ecology
GACR (19-05048S): 2019-2021 Role of exosomes in information transfer between cancer-associated fibroblasts and cancer cell, with emphasis on malignant melanoma
Deadline is closed