Project: Interplay of cytoskeletal organizers and membrane trafficking in root tip development
Research group: Cvrčková
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Recently, we documented that AtFH1, a membrane-bound cytoskeletal organizer from the formin family, undergoes controlled intracellular relocalization in the Arabidopsis root tip, such as early localization to developing plasmodesmata and transient labelling of endomembranes including the tonoplast. Functional importance of these changes remains unknown. We shall use the unique properties of the seedling root tip, whose developmental zonation allows simultaneous observation of tissues of varying age, to establish the developmental sequence of structural events (such as plasmodesma development or vacuole fusion) and localization of FH1 and other plasmodesma- or endomembrane- localized proteins (including proposed new plasmodesma components identified in the laboratory). Distinct protein localization events will be mapped with respect to anatomical landmarks of root development (e.g. first emerging root hairs). Co-expression of fluorescent protein-tagged versions of the proteins of interest carrying different fluorochromes will be used to identify invariable sequences of protein localization with respect to each other and to developmental events. The role of the cytoskeleton and of selected signalling pathways related to membrane trafficking in the observed developmental and protein localization processes will be examined using mutants in specific genes and pharmacological intervention. The results should bring novel insight into the cellular mechanisms of root development.
Deadline is closed