Whole genome duplication (polyploidization) is a key diversity-shaping force in plants but we still know little about its ecological and genomic consequences in natural populations. Cardamine amara is a predominantly European species encompassing multiple diploid and tetraploid lineages with different distribution, ecology and morphology representing excellent model of incipient speciation. Its closest relatives from the C. amara group, also in focus of the project, occur in Mediterranean, Anatolia, Caucasus and the Far East Asia. The following questions will be addressed in the PhD project, using the combination of the high-throughput sequencing (HybSeq, RADseq and whole genome resequencing), cytometry, GISH, chromosome painting, ecological niche and morphometric analyses: What was the role of (parallel) ecological adaptation in diversification of the taxa of the C. amara group? Is hybridisation among the taxa of the C. amara group linked with polyploidization, leading to formation of novel allopolyploid lineages? What is the strength of realized gene flow between diploid and tetraploid populations within the species? Had the genome of C. amara contributed to the formation of the Mediterranean and Eastern Asian C. amara group polyploids? What is the structural stability of parental subgenomes in selected allopolyploids as revealed by GISH/comparative chromosome painting?
Monnahan P*, Kolář F*, Baduel P*, Sailer C, Koch J, Horvath R, Laenen B, Schmickl R, Paajanen P, Šrámková G, Bohutínská M, Arnold B, Weismann C, Marhold K, Slotte T, Bomblies K, Yant L: Pervasive population genomic consequences of genome duplication in Arabidopsis arenosa. BiorXiv preprint.
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Novikova PYu, Hohmann N, Nizhynska V, Tsuchimatsu T, Ali J, Muir G, Guggisberg A, Paape T, Schmid K, Fedorenko OM, Holm S, Säll T, Schlötterer Ch, Marhold K, Widmer A, Sese J, Shimizu KK, Weigel D, Krämer U, Koch MA, Nordborg M, 2016: Sequencing of the genus Arabidopsis identifies a complex history of nonbifurcating speciation and abundant trans-specific polymorphism. – Nature Genetics 48: 1077–1082. https://dx.doi.org/10.1038/ng.3617
How hybridisation and allopolyploidisation affect the diversity in three Brassicaceae tribes (GAČR – Czech Science Foundation; 2019-2021)
GAČR – Czech Science Foundation grant
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