Our lab aims to solve medically relevant basic biological questions, focusing on Non-canonical Notch signaling and Wnt/Notch crosstalk in development, homeostasis and disease.

We strive to achieve a better mechanistic insight into the processes co-regulated by direct protein-protein interaction between components of Notch and Wnt pathways and Non-canonical Notch ligand signaling. To achieve this, we use CRISPR/Cas9 based technologies, together with scRNA seq and MS-Chip omics in mouse and human cell lines, as well as manipulation of relevant genes in the mouse model.

Aim of the proposed projects is to functionally test the signaling roles of the Notch ligand Jagged 1 intracellular domain (J1ICD) in mammals.

Projects take advantage of the known Allagile syndrome-causing mutations in J1ICD (1), and recapitulate them in vivo using mouse models to understand their pathophysiological role in development. We will apply the newly developed animal/cell tools to search for novel binding partners of J1ICD, and gene targets using MS-Chip technology. Describing a Notch ligand cis-signaling bears the potential to fundamentally change the field of Notch biology.

Students will be encouraged and supported in seeking additional fellowship funding and collaborative projects abroad. Position will be funded by PRIMUS grant.

Techniques: confocal microscopy, gene targeting, mouse in vivo experiments, cloning, WB, ISH immunoprecipitation, sc-RNA-seq, MS-Chip.

Requirements: Msc. student with a background in developmental or molecular biology, experience with cell cultures/animal handling/bioinformatics is beneficial.

Relevant publication: J. Mašek, E. R. Andersson, The developmental biology of genetic Notch disorders. Development. 144, 1743–1763 (2017).

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